Phytochemical screening and analysis of selected medicinal. Medicinal plants continue to provide valuable therapeutic agents, both in modern and traditional medicine 3. Phytochemical screening and gcms studies of the medicinal plant. In vitro antidiabetic potential and gcms analysis digera. Cyperaceae s srinivasan and v priya abstract in the present study the investigation on phytochemical screening was carried along with gc ms analysis to identify the phytoconstituents present in extracts of cyperus dubius, rottb. Hence, the present study is carried out using gcms analysis for phytochemical components from ethanolic extract of pseudoglochidion anamalayanum gamble. Keywords medicinal plants metabolomics gcms lcms flavonoids genistein. Medicinal plant, phytochemical screening, uvvisible. Gcms analysis was performed to identify some of the potent. Introduction medicinal plants have been used for countries as remedies for human disease 1. Phytochemical analysis phytochemical screening of crude extracts of pergularia daemia was carried out according to the methods described by trease and evans 15,16.
Raj ms, kameshwari mn, tharasaraswath kj, shubharani r 2017 qualitative and. Then filtrate was centrifuged at 2500 rpm for 15 minutes and filtrate was stored in sterile bottles at 5 0c for further use harbone, 1973. Phytochemical screening and gcms, ftir analysis of. Phytochemical screening and gc ms analysis of methanolic extract of an endemic doi. In this modern era, medicinal plants are at great attention to the researchers as most of the drug. The name of the compound, retention time and the percentage of their presence indicated as peak area are listed in tables 14, respectively, for the four plants extracts. The phytochemical screening showed the presence of alkaloids, tannins, steroids, flavonoids, saponins, anthraquinones, cardiacglycosides, phlobatinnins and carbohydrates. Phytochemical screening, gcms, ftir analysis of methanolic. Sagittaria trifolia is a medicinal foodstuff of china and east asia belonging to the. The gcms analysis of the acetone extract revealed the presence of five major compounds.
Gcms analysis of phytochemical compounds pertanika j. Eclipta prostrata is a perennial herb, distributed in the tropical and sub tropical regions of the world. Phytochemical screening and gcms analysis of mukia. Nearly 12 compounds were indentified in the pantnagar sample. Phytochemical composition of potentilla anserina l. Comparative phytochemical analysis of cuscuta reflexa roxb. Phytochemical characterization and comparative studies of.
Lamiaceae by gcms method shubhangi nagorao ingole department of botany, bar. Phytochemical analysis and assessment of antioxidant and. Gcms analysis of bioactive compounds in methanolic extract of. Phytochemical screening and gc ms analysis of cyperus dubius, rottb. Phytochemical screening and gcms studies of the medicinal. Gcms analysis of phytocomponents in the ethanol extract. Gcms analysis methanol extract of solanum torvum leaves was analyzed with the help of gcms analyzer perkin. Phytochemical analysis of a folklore medicinal plant citrullus colocynthis l bitter apple. The results also indicated potential antioxidants as well as antimicrobial activities against pdr s. Principle and application of gcms detector in phytochemical analysis gcms plays a key role in the analysis of unknown components of plant origin. Gcms analysis of phytochemical compounds in aqueous leaf.
Elite db 5m column and the gcms solution version 2. The phytochemical analysis showed that the ephedra intermedia plant extract contains a mixture of phytochemicals as reducing sugars, cardiac glycoside, phenolic compounds, flavonoids, and alkaloids. Gcms analysis was performed on the methanolic extract of s. Phytochemical screening and gcms analysis of cyperus. Introduction the indian subcontinent is rich in medicinal plants and is one of the richest countries in terms of genetic diversity of medicinal plants. The gcms analysis revealed the presence of 16 major compounds, with. In recent years gcms studies have been increasingly applied for the analysis of medicinal plants as this technique has proved to be a reliable method for the analysis of nonpolar components. Phytochemical analysis was carried out for identification of tannins, terpenoids, flavonoid, alkaloid, phenol, phytosterol and saponins according to standard methods trease and evans.
The name of the compound, retention time and the percentage of their presence indicated as peak area are listed in tables 14, respectively, for the four plants. Gas chromatographymass spectrometry gcms analysis of the whole plant. Phytochemical, gcms analysis and antibacterial activity of a medicinal plant acalypha indica. Pdf phytochemical analysis of some medicinal plants. Phytochemical analysis of methanolic extracts of leaves of. The phytochemical profile of methanolic leaf extract from cecropia species was further explored using an hplc method coupled to both dad and ms detectors. Graphical representation of data was performed by using sigma plot 10. Phytochemical and gcms analysis of bioactive compounds of. Preliminary phytochemical screening and gcms analysis of methanol extract of ceropegia pusilla 51 water bath and it was filtered through whatman no. Phytochemical screening and gcms analysis of methanolic.
Gcms analysis of phytochemical compounds of normal and leaf. Gcms chromatogram of methanolic extracts of cuscuta reflexa muzaffarnagar sample. Gcms analysis of this extract was performed using a perkin elmer gc claurus 500 system and gas chromatograph interfaced to a mass spectrometer gcms equipped with a elite1 fused silica capillary column 30 m. Phytochemical screening and gcms analysis of methanolic leaf. Department of chemistry, institute of science, civil lines, nagpur 440001, maharashtra, india. Phytochemical analysis of the methanolic extracts of nine egyptian plants confirmed that these extracts were rich in phenolics, flavonoids, and alkaloids.
Phytochemical screening phytochemical analysis was carried out for identification of terpenoids, flavonoid, tannins, phenols, phytosterols, alkaloids and saponins according to standard methods kumar et al. The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the e. The crude extract form was subjected to column chromatography over silica gel 100200 mesh and eluted with nhexane, chloroform, ethanol. Pharmacognosy gcms phytochemical analysis day is international event on 11 october, every year and commonly known as pharmacognosy gcms phytochemical analysis fair which provide leading platform to business activities in pharmacognosy gcms phytochemical analysis, sales and purchase of instruments, and services. Phytochemical analysis was carried out for identification of tannins, terpenoids, flavonoid, alkaloid, phenol, phytosterol and saponins according to standard methods trease and evans, 2008. Preliminary phytochemical screening and gc ms issn profiling. Phytochemical analysis of leaf extract of ocimum americanum l. The gcms analysis of the essential oil of the seeds identified the presence of. An endangered medicinal plant mohan b, nayak jb, r sunil kumar, lp shiva kumari, mohan ch and rajani b. Phytochemical analysis of crude powder of the samples for the evaluation of phytochemicals. An experience from north east india j pharm res phytochemical analysis of traditional medicinal plants and their antimicrobial activity. Gcms analysis of the essential oil and methanol extract.
The compounds present in the hydroalcoholic extracts of mukia maderaspatana were identified by. Phytochemical screening, gcms analysis of decalepis. Journal of medicinal plants studies phytochemical and find the in vitro antidiabetic activity of. Phytochemical and gcms analysis of canthium coromandelicum.
Preliminary phytochemical screening and gc ms analysis of methanol extract of ceropegia pusilla 51 water bath and it was filtered through whatman no. Hence, the present study is carried out using gcms analysis for phytochemical components from ethanolic extract of. Calotropis gigantea, phytochemical screening, gcms analysis. A study of phytochemical constituents in caralluma umbellata. Principle and application of gc ms detector in phytochemical analysis gc ms plays a key role in the analysis of unknown components of plant origin. Natural phytochemicals contained in plants, such as alkaloids, flavonoids. An endangered medicinal plant mohan b, nayak jb, r sunil kumar, lp shiva kumari, mohan ch and rajani b abstract the present paper reports the phytochemical and gc ms analysis studies of an endemic endangered, climbing shrub. Medicinal plants have been used for centuries as remedies for human disease. Acalypha indica distributed in the southern part of india particularly in tamilnadu and it has potential medicinal properties and used as diuretic, anthelmintic and for respiratory problems such as bronchitis, asthma and pneumonia. Antimicrobial and phytochemical analysis of leaf extract of medicinal fruit plants. Preliminary phytochemical screening, quantitative analysis of. The gc ms chromatogram of these medicinal plant extracts was shown in the figure 3.
Research article gcms analysis of phytochemical compounds present in the rhizomes of nervilia aragoana gaud elizabeth thomas1, aneesh t. Preliminary phytochemical screening, quantitative analysis. Gcms analysis of phytocostituents of some wild zingiberaceae. An experience from north east india j pharm res phytochemical analysis of traditional medicinal plants. Phytochemical analysis of species of the genus cecropia the polyphenolic content of four authentic plant species and commercial products of the genus cecropia species 15 samples were analized. The peaks of the compounds with retention time in mins identified by gcms analysis of methanolic extracts of four plants respectively figure. Antioxidant activity was analyzed by using 2,2diphenyl1picrylhydrazylhydrate assay. C nielsen from lampung, indonesia irawan candra 1 foliatini dr. It exhibits a wide range in topography and climate. Phytochemical analysis and gcms analysis of leaves of. A study of phytochemical constituents in caralluma.
For the widespread acceptance of herbal medicines, standardization, quality control of the herbal materials, as well as. Phytochemical screening and gcms analysis of acetone. Phytochemical analysis, molecular docking and molecular. Gc ms total ion chromatogram of the methanol extract of the leaves of kayea assamica table 2. Phytochemical analysis of leaf extract of eclipta alba l.
To investigate the importance of any medicinal plant, the initial or first. Gcms analysis of the plant extracts was performed by using gcms equipped with a. Phytochemical screening and gcms studies of the medicinal plant pavetta indica. Gcms analysis of bioactive phytochemicals present in ethanolic. Gcms analysis of the essential oil and methanol extract of. It includes auto sampler and a gas chromatograph which interfaced to a mass spectrophotometer. India is the largest producer of medicinal herbs and is appropriately called the. Phytochemical analysis, antioxidant, antistress, and. Phytochemical screening of the entire herb of lactuca runcinata dc revealed the.
Alangium salviifolium, phytochemical, antibacterial, gcms introduction in this present era, many natural product researchers has focused on the plant research in all over the world and a wealth of evidence shows immense potential of ethnomedicinal plants. Gc ms analysis methanol extract of solanum torvum leaves was analyzed with the help of gc ms analyzer perkin. Therefore, search for newer drugs with minimum side effects obtained from. Preliminary phytochemical screening and gc ms issn. The crude extract form was subjected to column chromatography over silica gel 100200 mesh and eluted with nhexane, chloroform, ethanol and methanol respectively. Jabin f, nasreen s 2016 phytochemical analysis of some medicinal plants. Gcms analysis and phytochemical screening of a rare. India is the largest producer of medicinal herbs and is appropriately called the botanical garden of the world1,2.
Phytochemical analysis by gcms analysis of the plant rhizomes revealed the presence of different fatty acids, heterocyclic compounds etc. Rjpt gcms analysis of phytochemical compounds present in. Volatile compound analysis using gcms, phytochemical. Rjpt gcms analysis of phytochemical compounds present. Preliminary phytochemical screening of the extract was carried out according to the standard method described by brindha et al. Phytochemical analysis, gcms analysis, macrotyloma uniflorum, and oleic acid. Gcms analysis of phytocomponents in the ethanol extract of. Gcms analysis of phytochemical compounds in aqueous. Phytochemical, gcms analysis and antibacterial activity. Raj ms, kameshwari mn, tharasaraswath kj, shubharani r 2017 qualitative and quantitative analysis of phytochemicals in two different species of urginea. The column was fused silica capillary, hp5 column 30. To conduct a gcms analysis on ethanolic leaves extracts 25 com.
Gcms analysis of phytochemical compounds of normal and. Gc ms analysis of cassia italica leaf methanol extract asian j pharm clin res. Phytochemical screening and gas chromatographymass. An experience from north east india minakshi b, jharna d, chanbi devi e, nayan t, partha pk, kundal n. The methanol fractions of each plant were taken for gc ms analysis. Phytochemical screening and gcms analysis of hexanolic extract of lantana camara linn mamta uparkar and sunil h. Gc ms chromatogram of methanolic extracts of cuscuta reflexa muzaffarnagar sample. Phytochemical, gcms analysis and antibacter ial activity of a medicinal plant acalypha indica s. Lib libraries provided by the software of the gcms system turbomass.
Phytochemical analysis by gcms analysis of the plant rhizomes revealed the presence of different fatty acids, heterocyclic. The detailed tabulations of gcms analysis of the extracts are given in table 1, table 2, table 3, table 4 and table 5 respectively. Oct 21, 2014 phytochemical analysis, antioxidant, antistress, and nootropic activities of aqueous and methanolic seed extracts of ladies finger abelmoschus esculentus l. In recent years gcms studies have been increasingly applied for the analysis of medicinal plants as this technique has proved to be a reliable method for the analysis of nonpolar components and volatile essential oil, fatty acids, lipids and alkaloids 89. Multivariate statistics of the integrated gcms and lcms data showed. Gc ms analysis of phytochemical compounds pertanika j. Phytochemical analysis of traditional medicinal plants and their antimicrobial activity. Introduction plants are used as a source of medicine that has been inherited as one of the important component of the health care system. Phytochemical analysis and assessment of antioxidant and antimicrobial activities of some medicinal plant species from egyptian flora. Phytochemical analysis in different extracts methanol, hexane revealed the presence of secondary metabolites like.
Pdf phytochemical studies and gas chromatographymass. Gc ms ionizes compounds and measures their mass numbers. Plants have a great potential for producing new drugs of great benefit to mankind. Pharmacognosy gcms phytochemical analysis day is international event on 11 october, every year and commonly known as pharmacognosy gcms phytochemical analysis fair which provide leading platform to business activities in pharmacognosy gcms. Phytochemical, gcms analysis and antibacterial activity of a. The phytochemical screening and gcms analysis of the extracts are in good agreement with the presence of alkaloids. Medicinal plants are the most potent antimicrobial natural source. Phytochemical analysis, biochemical and mineral composition and. Phytochemical analysis of traditional medicinal plants and. Phytochemical screening and gcms analysis of leaf extract.
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